TOTAL EXPOSURE! P1. Generation of SARS-CoV-2 Spike Pseudotyped Virus for Viral Entry and Neutralization and their patents, vaccine patents

I think it's all pretty much over my head but I can put two and two together for you and match it with the patents

79.74% of these words I've never seen before made it all the more interesting After setting out on my search the other day on the enzyme computer components in biological computing I wanted to see maybe the spike protein would be doing the same thing and this is what I found buckle up buttercup   ….. You might find it easier to read the whole thing before embarking on the patent lookup in this section you will find the Google patent domain link and where there is a domain link in the next segment part two you will find the patent In this study, i present an effective methodology for studying the role of the SARS-CoV-2 spike protein in determining cellular tropism and viral entry with potential applications for the screening of anti-SARS-CoV-2 neutralizing antibodies and alternative SARS-CoV-2 entry inhibitors The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus requires reliable assays for studying viral entry mechanisms which remains poorly understood. This knowledge is important for the development of therapeutic approaches to control SARS-CoV-2 infection by permitting the screening for neutralizing antibodies and other agents that can block infection (So they say) This is particularly important for patients who are at high risk for severe outcomes related to covid-19 The production of pseudotyped viral particles 

https://patents.google.com/?q=C12N15%2f86

            

https://patents.google.com/?q=C12N15%2f867

           

https://patents.google.com/?q=(16%2f28)&q=(C07K)&oq=+C07K+16%2f28+

         

https://patents.google.com/?q=C12N2740%2f15023

 

https://patents.google.com/?q=C12N7%2f00

                                       may seem like a daunting task for a non-virology laboratory without experience in the two most commonly used pseudotyping systems, namely retro/lentiviruses and vesicular stomatitis virus 

https://patents.google.com/?q=A61K35%2f76

     (VSV) which lacks the VSV envelope glycoprotein 

https://patents.google.com/?q=C12N2760%2f20222

  (VSVΔG). By incorporating the most up-to-date knowledge, they have developed a detailed, easy-to-follow novel protocol for producing SARS-CoV-2 spike-bearing pseudovirus using the VSV-ΔG system. 

https://patents.google.com/?q=A61K2039%2f53

    

https://patents.google.com/?q=C07K16%2f10

       They describe the infection assay which uses GFP fluorescence as a measure of infection in a 24-well live imaging system. 

https://patents.google.com/?q=G01N21%2f6486

                                They present results of the optimization of the system to enhance viral infection 

https://patents.google.com/?q=G01N33%2f56983

  levels through the over-expression of human ACE2 receptor and the overexpression of at least one of two proteases -

https://patents.google.com/?q=G01N33%2f577

           

https://patents.google.com/?q=(14%2f705)&q=(C07K)&oq=+C07K+14%2f705

    

https://patents.google.com/?q=A61P31%2f14

                            TMPRSS2 or Furin, as well as, supplementation with Poloxamer 407 Poloxamer 407 is a hydrophilic non-ionic surfactant of the more general class of copolymers known as poloxamers.    

https://patents.google.com/?q=C08L71%2f02

        (P407)Mucosal Applications of Poloxamer 407-Based Hydrogels: An Overview https://www.semanticscholar.org/paper/Mucosal-Applications-of-Poloxamer-407-Based-An-Giuliano-Paolino/b1f3179264d0ed9cd250f2f07d08ba6c80fd8585 

https://patents.google.com/?q=A61K9%2f0019

  and Prostaglandin E2 (PGE2) as adjuvants. 

https://patents.google.com/?q=C07K16%2f468

       The spike protein of SARS-CoV-2   

https://patents.google.com/?q=C07K14%2f005

      contains the furin protease   

https://patents.google.com/?q=C12P21%2f06

  recognition site and its cleavage enhances the infectivity of this virus. The binding of SARS-CoV-2 to the ACE2 receptor  

https://patents.google.com/?q=G01N33%2f573

  has been shown to downregulate ACE2, thereby increasing the levels of pathogenic angiotensin II (Ang II).  

https://patents.google.com/?q=A61K38%2f085

  

https://patents.google.com/?q=A61K45%2f06

  Furin is a protease, a proteolytic enzyme 

https://patents.google.com/?q=A61K38%2f47

 activated by substrate presentation that in humans and other animals is encoded by the FURIN gene. TMPRSS2 (mCherry)] Human ACE2  

https://patents.google.com/?q=C12N9%2f485

  was cloned into pDEST-mCherry vector with an N-terminal mCherry tag. TMPRSS2 and TMPRSS4           pDest-mCherry-N1 (Plasmid #31907) ; Purpose. Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli.  

https://patents.google.com/?q=C12N15%2f66

 

https://patents.google.com/?q=C07K16%2f40

       mCherry is a bright red monomeric fluorescent protein 

https://patents.google.com/?q=C07K14%2f43595

      created by rounds of directed evolution of DsRed  

https://patents.google.com/?q=C12Q1%2f37

  in my previous article about turning people into biological computers  

Cancer, Enzyme, parasite biological computers your DNA the human body and the vaccine bio weapon connection nanobiotechnology

using enzymes it talked about fluorescence and how they are ones and zeros in computer binary , Synthetic genetic circuits offer the potential to wield computational control over biology, but their complexity is limited by the accuracy of mathematical models. Here, they present advances that enable the complete encoding of an electronic chip in the DNA carried by Escherichia coli (E. coli).    https://patents.justia.com/patents-by-us-classification/435/849                   Methods for generating barcoded combinatorial libraries  https://www.osti.gov/biblio/1568324 The chip is a binary‐coded digit (BCD) to 7‐segment decoder, associated with clocks and calculators, to turn on segments to visualize 0–9. Design automation is used to build seven strains, each of which contains a circuit with up to 12 repressors and two activators (totaling 63 regulators and 76,000 bp DNA). The inputs to each circuit represent the digit to be displayed (encoded in binary by four molecules), and output is the segment state, reported as fluorescence   https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058928/ TMPRSS2 (mCherry)contains a preparation of Homo sapiens embryonic kidney epithelial cells (HEK-293T aborted baby) which were modified to express the transmembrane protease,   

https://patents.google.com/?q=C12N9%2f6424

 serine 2 mCherry, mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. https://en.m.wikipedia.org/wiki/MCherry   takes advantage of the strong Ptac promoter, 

https://patents.google.com/?q=C12N15%2f72

  which has been shown to function in many bacterial species, 

https://patents.google.com/?q=C12Q1%2f689

  and eliminates the need for adding an inducing substance for protein expression.Two hybrid promoters functional in Escherichia coli were constructed. These hybrid promoters, tacI and tacII, were derived from sequences of the trp and the lac UV5 promoters. 

https://patents.google.com/?q=C12N15%2f72

      

https://patents.google.com/?q=C12P21%2f02

    In the first hybrid promoter (tacI), the DNA upstream of position –20 with respect to the transcriptional start site was derived from the trp promoter. The DNA downstream of position –20 was derived from the lac UV5 promoter. In the second hybrid promoter (tacII), the DNA upstream of position –11 at the Hpa I site within the Pribnow box was derived from the trp promoter. The DNA downstream of position –11 is a 46-base-pair synthetic DNA fragment that specifies part of the hybrid Pribnow box  

https://patents.google.com/?q=C12P21%2f02

   and the entire lac operator. 

https://patents.google.com/?q=C12N15%2f72

  It also specifies a Shine–Dalgarno sequence flanked by two unique restriction sites (portable Shine–Dalgarno   

https://patents.google.com/?q=C07K14%2f56

 sequence). https://pubmed.ncbi.nlm.nih.gov/6337371/ (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2) do you have any clue how many times we see Ace 2 in patents now if enzymes 

https://patents.google.com/?q=A61K8%2f66

    are being used as biological computer components well I'm starting to think the whole COVID scam is nothing but turning the human body into a biological computer.THE BIOLOGICAL MICROPROCESSOR, OR HOW TO BUILD A COMPUTER WITH BIOLOGICAL PARTS  https://www.sciencedirect.com/science/article/pii/S200103701460026X     

https://patents.google.com/?q=C08L2203%2f02

                       

https://patents.google.com/?q=B82Y5%2f00

               

https://patents.google.com/?q=H04B13%2f005

    Classifications

H04R25/554 Deaf-aid sets, i.e. electro-acoustic or electro-mechanical hearing aids; Electric tinnitus maskers providing an auditory perception using an external connection, either wireless or wired using a wireless connection, e.g. between microphone and amplifier or using Tcoils

G06F16/951 Indexing; Web crawling techniques

H04B13/005 Transmission systems in which the medium consists of the human body

H04K1/00 Secret communication

H04L63/08 Network architectures or network communication protocols for network security for authentication of entities

H04L9/3231 Biological data, e.g. fingerprint, voice or retina

H04R3/00 Circuits for transducers, loudspeakers or microphones

H04L2209/805 Lightweight hardware, e.g. radio-frequency identification [RFID] or sensor

H04R2460/13 Hearing devices using bone conduction transducers      Classifications

H04Q9/04 Arrangements for synchronous operation

           TMPRSS2 A TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic https://www.nature.com/articles/s41586-022-04661-w    is an endothelial cell surface protein that is involved in the viral entry and spread of coronaviruses including severe acute respiratory syndrome  https://www.rcsb.org/search?request=%7B%22query%22%3A%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22terminal%22%2C%22service%22%3A%22full_text%22%2C%22parameters%22%3A%7B%22value%22%3A%22Corona%20virus%22%7D%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%5D%2C%22logical_operator%22%3A%22and%22%2C%22label%22%3A%22full_text%22%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%2C%22return_type%22%3A%22entry%22%2C%22request_options%22%3A%7B%22paginate%22%3A%7B%22start%22%3A0%2C%22rows%22%3A25%7D%2C%22results_content_type%22%3A%5B%22experimental%22%5D%2C%22sort%22%3A%5B%7B%22sort_by%22%3A%22score%22%2C%22direction%22%3A%22desc%22%7D%5D%2C%22scoring_strategy%22%3A%22combined%22%7D%2C%22request_info%22%3A%7B%22query_id%22%3A%22e2482d1a37b3d3a51e398d2938ff5d72%22%7D%7D The TMPRSS2 gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a low density. The TMPRSS2 gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a low density.. Clan PA proteases bearing the trypsin fold  

https://patents.google.com/?q=C12N9%2f52

  are the largest family of serine proteases and perhaps the best studied group of enzymes. Digestive enzymes such as trypsin  

https://patents.google.com/?q=C12N9%2f6427

  and chymotrypsin cleave polypeptide chains at positively charged (Arg/Lys) or large hydrophobic (Phe/Trp/Tyr) residues, respectively.    The coronavirus disease (COVID-2019) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) https://www.rcsb.org/search?request=%7B%22query%22%3A%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22terminal%22%2C%22service%22%3A%22full_text%22%2C%22parameters%22%3A%7B%22value%22%3A%22sars%20%5C%22cov-2%5C%22%22%7D%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%5D%2C%22logical_operator%22%3A%22and%22%2C%22label%22%3A%22full_text%22%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%2C%22return_type%22%3A%22entry%22%2C%22request_options%22%3A%7B%22paginate%22%3A%7B%22start%22%3A0%2C%22rows%22%3A25%7D%2C%22results_content_type%22%3A%5B%22experimental%22%5D%2C%22sort%22%3A%5B%7B%22sort_by%22%3A%22score%22%2C%22direction%22%3A%22desc%22%7D%5D%2C%22scoring_strategy%22%3A%22combined%22%7D%2C%22request_info%22%3A%7B%22query_id%22%3A%2250cf45f67dcb7ff8ec823fdaeb0aacea%22%7D%7D   SARS-CoV-2 is an enveloped, positive-sense single stranded RNA virus belonging to the Betacoronavirus genus of the Coronaviridae family of viruses Understanding the viral entry process is key for the development of therapeutic strategies to prevent viral spread. The spike (S) glycoprotein is present on the surface of SARS-CoV-2 and is responsible for the viral tropism through host cell recognition by binding to the human angiotensin-converting enzyme 2 (hACE2) receptor    Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo virus  A trunked radio system is a two-way radio system that uses a control channel to automatically assign frequency channels to groups of user radios, does that apply here I don't know.   what is a VSV pseudo virus   https://www.rcsb.org/search?request=%7B%22query%22%3A%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22terminal%22%2C%22service%22%3A%22full_text%22%2C%22parameters%22%3A%7B%22value%22%3A%22VSV%20pseudo%20virus%22%7D%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%5D%2C%22logical_operator%22%3A%22and%22%2C%22label%22%3A%22full_text%22%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%2C%22return_type%22%3A%22entry%22%2C%22request_options%22%3A%7B%22paginate%22%3A%7B%22start%22%3A0%2C%22rows%22%3A25%7D%2C%22results_content_type%22%3A%5B%22experimental%22%5D%2C%22sort%22%3A%5B%7B%22sort_by%22%3A%22score%22%2C%22direction%22%3A%22desc%22%7D%5D%2C%22scoring_strategy%22%3A%22combined%22%7D%2C%22request_info%22%3A%7B%22query_id%22%3A%220108b0ab19fa4c1e9dfa18e6fc707a5b%22%7D%7D Rrecent studies demonstrated that the spike glycoprotein from SARS-CoV-2 can be “pseudotyped” onto replication defective viral particles such as HIV-based lentiviral particles, murine leukemia virus (MLV)-based retroviral particles, or Vesicular Stomatitis Virus Classifications

C12N15/86 Viral vectors

A61K48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

C12N2740/16043 Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

C12N2740/16052 Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

C12N2830/00 Vector systems having a special element relevant for transcription

C12N2830/15 Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination

C12N2830/50 Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

C12N2830/60      a technology hiv-1, which is applied in the field of molecular biology and virology HIV is the most studied lentivirus, and the predominant primary parent virus from which currently available lentiviral vectors  The differential characteristics of lentiviral vectors based on human and simian immunodeficiency viruses (HIV and SIV)  Vector systems having a special element relevant for transcription from viruses  (VSV)    Three gene therapy strategies have received US Food and Drug Administration (FDA) approval; one includes HIV-1-based lentiviral vectors. These vectors incorporate features to provide long-term gene transfer and expression while minimizing generation of a replication-competent virus or pathogenicity https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(21)00058-9 https://www.frontiersin.org/articles/10.3389/fviro.2021.793320/full  Both fLuc and SEAP provide a wide dynamic range when analyzing inhibitors of infection (either antiviral or neutralizing antibodies).   rVSVΔG*Reporter can be packaged and released from cells transfected with mammalian expression plasmid encoding G protein or the envelope protein from other viruses .The spike (S) glycoprotein of SARS-Cov-2 facilitates viral entry into target cells via the cell surface receptor angiotensin-converting enzyme 2 (ACE2). Third generation HIV-1 lentiviral vectors can be pseudotyped to replace the native CD4 tropic envelope protein of the virus and thereby either limit or expand the target cell population https://www.frontiersin.org/articles/10.3389/fviro.2021.793320/full              Human coronaviruses (CoV) are enveloped, positive stranded RNA viruses of the family of Coronaviridae  

https://patents.google.com/?q=C07K16%2f1002

 https://www.rcsb.org/search?request=%7B%22query%22%3A%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22group%22%2C%22nodes%22%3A%5B%7B%22type%22%3A%22terminal%22%2C%22service%22%3A%22full_text%22%2C%22parameters%22%3A%7B%22value%22%3A%22Coronaviridae%22%7D%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%5D%2C%22logical_operator%22%3A%22and%22%2C%22label%22%3A%22full_text%22%7D%5D%2C%22logical_operator%22%3A%22and%22%7D%2C%22return_type%22%3A%22entry%22%2C%22request_options%22%3A%7B%22paginate%22%3A%7B%22start%22%3A0%2C%22rows%22%3A25%7D%2C%22results_content_type%22%3A%5B%22experimental%22%5D%2C%22sort%22%3A%5B%7B%22sort_by%22%3A%22score%22%2C%22direction%22%3A%22desc%22%7D%5D%2C%22scoring_strategy%22%3A%22combined%22%7D%2C%22request_info%22%3A%7B%22query_id%22%3A%22ed06fcaa86b1e595e8fda9dc22ef6acb%22%7D%7D      Introduction: NGOs and governments of some countries have demanded suspension of patents protection of COVID-19 vaccines and the underlying technology to enhance worldwide access. At the same time, companies actually developing and producing COVID-19 vaccines have to navigate the patent landscape and have to deal with 3rd party patents.  https://tandf.figshare.com/articles/dataset/The_patent_maze_of_COVID_19_vaccines/15051936            WHO COVID-19 Technology Access Pool   https://www.who.int/initiatives/covid-19-technology-access-pool     Patents for Humanity: COVID-19 category award recipients   https://www.uspto.gov/ip-policy/patent-policy/patents-humanity/patents-humanity-covid-19-category-award-recipients       

Patents Prove SARS-CoV-2 Is A Manufactured Virus   https://m.vk.com/@g.tatari-patents-prove-sars-cov-2-is-a-manufactured-virus       

https://patents.google.com/?q=B82Y40%2f00

                                              Protein-based nano-‘computer’ evolves in ability to influence cell behavior   https://www.psu.edu/news/research/story/protein-based-nano-computer-evolves-ability-influence-cell-behavior/     Generation of SARS-CoV-2 Spike Pseudotyped Virus for Viral Entry and Neutralization Assays: A 1-Week Protocol  https://www.frontiersin.org/articles/10.3389/fcvm.2020.618651/full Ghg  Classifications

A61K39/12 Viral antigens

A61K39/215 Coronaviridae, e.g. avian infectious bronchitis virus

A61P31/14 Antivirals for RNA viruses

C07K14/005 Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses

C12N7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof

C12N2770/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

C12N2770/20034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

C12N2770/20071 Demonstrated in vivo effect Classifications

G01N33/56983 Viruses

G01N2333/165 Coronaviridae, e.g. avian infectious bronchitis virus

G01N2469/20 Detection of antibodies in sample from host which are directed against antigens from microorganisms

Isaiah 54:17"No weapon that is fashioned against you shall prosper..." "No weapon that is fashioned against you shall prosper, and you shall confute every tongue that rises against you in judgment. This is the heritage of the servants of the Lord and their vindication from me, says the Lord Love, Repentance, Faith, Obedience, Purpose, Fearless, and Prayer Thanks for all that you do to stop this biohack on all that is natural and made in the image of GOD and may Romans 8:31 be our guiding light. 6831